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Genetic / Molecular Profiling Core

This REACT Center and UAB-affiliated Core provides expertise related to research support in genetic and molecular profiling.

To request more information about the services listed below or if you need a service not listed here, please contact us or call (205) 996-4086.

Bioanalytical Redox Biology Core

Promotes redox biology in diabetes-related research, emphasizing that diabetes is both a metabolic and a vascular disease. It serves as a hub to facilitate and promote the development of multi-disciplinary research projects among independently funded research programs in diabetes. The core promotes research in areas common to the metabolic and vascular aspects of diabetes and cardiovascular disease. It provides state-of-the-art services in mitochondrial damage, function, proteomics, and oxidative stress assessment support for investigators carrying out diabetes mellitus (DM) and cardiometabolic disease (CMD) research at UAB.

Comprehensive Flow Cytometry Core

The core’s mission is to provide state-of-the-art cell sorting and analytical flow cytometry services to support research programs.

Core Muscle Research Laboratory

The Core Muscle Research Laboratory is equipped and staffed to provide cellular, molecular, and tissue analysis of human muscle and blood biospecimens including histology, immunofluorescence and light microscopy, RT-PCR/in situ hybridization, immunoblotting, tissue culture, spectrophotometry, fluorometry, and digital darkroom imaging, along with in vitro experiments using stem cells and other cell types isolated from human biospecimens.

Heflin Center Genomics Core Lab

The Heflin Genomics Core offers genetic and genomic services including single nucleotide polymorphism detection, standard Sanger Sequencing and Next-Generation sequencing for multiple assays. The Next-Generation assays include whole exome profiling, whole transcriptome profiling, microRNA sequencing, Methylation sequencing, custom capture and sequencing, and microbiome/metagenomic sequencing.

Mass Spectrometry/Proteomics Shared Facility

Offers access to capabilities that are not available in individual laboratories.The facility is organized into five modules supported by highly trained scientists who are experts in mass spectrometry, bioanalytical chemistry, statistics, systems biology, and information handling. The facility’s goal is to provide state-of-the-art capabilities in mass spectrometry, proteomics, and bioanalytic technologies to support the research needs of UAB internal and external researchers.

Multidisciplinary Molecular Interaction Core

Supports a GE Biacore T200 optical biosensor instrument which employs surface plasmon resonance (SPR) technology for monitoring label-free real-time biomolecular interactions between macromolecules such as proteins, nucleic acids, carbohydrates, and lipids as well as small molecules. The instrumentation provides determinations of binding specificities; kinetics; affinity; concentration determination, and epitope mapping. The core is available on a fee-per-use basis, either with or without the assistance (for GE-trained users) of a dedicated expert operator of the equipment. Consultation and training are available.

Nanostring Laboratory

Housing the nCounter Analysis System by Nanostring Technologies, a platform which enables the profiling of hundreds of targets simultaneously with high sensitivity and precision, even in formalin fixed paraffin embedded (FFPE) tissues, without the need for amplification steps except in single cell or low cell number experiments. The system utilizes a novel digital technology that is based on direct multiplexed quantification of nucleic acids and provides highly reproducible data over five logs of dynamic range. Applications include gene expression analysis, microRNA and lncRNA analysis, copy number variation analysis, and ChIP-String analysis, and leukemia fusion gene analysis. Additionally, using the nCounter Elements Tagsets, users may assemble their own assays as part of development of a novel laboratory developed test.

Nanoscale Materials Fabrication and Characterization Core

Featuring state-of-the art nanofabrication and characterization facilities. The nanoscale materials and coatings can be composed of diamond, metals, ceramics, and polymeric materials for a variety of applications in biomedical implants, nanoparticles in bio-sensing, drug delivery, and biomedical imaging. These include facilities for the fabrication of nanostructured thin film coatings, nanoparticle synthesis, nanofibrous materials, and nanoscale patterning of surfaces using lithography.

Neuroscience Molecular Detection and Stereology Core

Assists researchers and provides training in all aspects of immunohistochemistry (IHC) and in situ hybridization (ISH) from proper fixation techniques and tissue processing, IHC/ISH protocol optimization, through final imaging and analysis. The Core specializes in amplification techniques for troublesome antibodies and multi-labeling techniques.

Neuroscience Vector and Vision Core

Offers expertise in both prokaryotic and eukaryotic expression systems, yeast two hybrid analyses, lentivirus and AAV production for gene expression, lentiviruses for gene repression (shRNA expression), reporter viruses (RFP, GFP, lacz), lentiviruses for CrispR/Cas9 mediated gene editing, and viruses for optogenetic probes.

Targeted Metabolomics and Proteomics Laboratory

Organized to provide a variety of analytical and technical services using mass spectrometry to investigators. The laboratory has three mass spectrometers, a triple quadrupole instrument (AB Sciex 4000), a quadrupole-linear ion trap instrument (AB Sciex 6500 Qtrap), and quadrupole-TOF (AB Sciex 5600 TripleTOF). The AB Sciex 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The combination of Eksigent microflow 200 LC and 415 nanoLC with the mass spectrometers provides the highest possible sensitivity for sample analysis.